mouse endothelial cells  (ATCC)

Journal: Nature Communications

Article Title: Environmental enrichment and physical exercise prevent stress-induced social avoidance and blood-brain barrier alterations via Fgf2

doi: 10.1038/s41467-025-68058-9

Figure Lengend Snippet: A , Experimental timeline for inflammatory insult with TNF-α and FGF2 co-treatment. Once confluent (4 days), HBEC-5i or bEnd.3 were pretreated with FGF2 or vehicle for one hour and then stimulated with TNF-α or vehicle for up to 7 days. FGF2 alters mouse ( B ) and human ( C ) endothelial transcription in response to acute TNF-α stimulation. FGF2 promotes faster restoration of TNF-α-induced Cldn5 loss in mouse ( D ) and human ( E ) endothelial cells. Chronic stimulation with TNF-α leads to a reduction in endothelial monolayer integrity measured by trans-endothelial electrical resistance (TEER) in mouse ( F ) and human ( G ) endothelial cells. FGF2 co-treatment preserves normal TEER despite TNF-α. H 7 days of TNF-α treatment promotes spikes and discontinuities in Cldn5 tight junction strands in bEnd.3, which is reversed by FGF2 treatment (scalebar = 20 μm). Data represent mean ± s.e.m., and each experiment was replicated at least twice on independent samples. Group comparisons were evaluated with two-way ANOVA followed by Bonferroni’s post hoc tests; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The human brain microvascular endothelial cell line HBEC-5i (ATCC CRL-3245, male donor according to https://www.cellosaurus.org/CVCL_4D10 ) and the mouse brain endothelial cell line bEnd.3 (ATCC CRL-2299) were subcultured and stored in banks at −150 ° C. Cells were thawed as needed and cultured in DMEM/F12 supplemented with 10% fetal bovine serum, 25 ug/mL gentamicin (Gibco), and 1X endothelial cell growth supplement (ScienCell).

Techniques:

Journal: Journal of Biological Engineering

Article Title: Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

doi: 10.1186/s13036-025-00593-x

Figure Lengend Snippet: Effect of coating agents on migration and gene expression of ECs cultured on PCLS. ( A ) Schematic diagram describes the experimental design of the EC migration assay using the decellularized PCLS. ( B , C ) Coating enhances the migration of Hoechst-labeled cell towards the PCLS compared to the uncoated group. ( D ) In a separate experiment, quantification of CLDN , CD34 , and eNOS gene expression was performed by quantitative real-time RT-PCR in C166 ECs seeded on PCLS for 24 h, showing increased gene expression in ECs grown within the coated PCLS compared to uncoated conditions. The results represent the mean of measurements taken from 3 technical replicates. Each number represents a biological replicate (independent experiment). Scale bar = 200 μm. Each group n = 3, mean ± SD, * p < 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: In this study, mouse endothelial cells (C166) (ATCC, Manassas, VA) were predominantly used.

Techniques: Migration, Gene Expression, Cell Culture, Labeling, Quantitative RT-PCR

Journal: Journal of Biological Engineering

Article Title: Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds

doi: 10.1186/s13036-025-00593-x

Figure Lengend Snippet: Re-endothelialization of decellularized mouse lung scaffolds. ( A ) Schematic diagram illustrating the strategy for re-endothelialization of the decellularized mouse lung scaffolds with C166 cells. ( B ) Hematoxylin and eosin (H&E) stained images and immunofluorescence images with 4’,6-diamidino-2-phenylindole (DAPI) (blue), laminin (green), and VE-cadherin (red) showed the cells were found to be randomly localized in the uncoated group, whereas in the coated groups, the cells were clearly lining blood vessels. Scale bar = 200 μm (top), 100 μm (middle), 100 μm (bottom). ( C ) Quantification of endothelial vessels per fields in each group. The results represent the mean of three images taken from each recellularized lung. Each number represents a biological replicate (independent whole lung scaffold). Each group n = 3, mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: In this study, mouse endothelial cells (C166) (ATCC, Manassas, VA) were predominantly used.

Techniques: Staining, Immunofluorescence